Professor of Biochemistry
Harvey Mudd College 1985
PhD, Cornell University 1991
NIH Postdoctoral Fellow, The Pennsylvania
Bioinorganic chemistry, protein folding, metal toxicology, metal NMR.
(757)-221-2558; e-mail: firstname.lastname@example.org
Research Focus & Highlights External Funding Publications Research Students Teaching Highlights
The major focus of my research effort at this time is development of new tools to investigate metal toxicology. Since heavy metals are persistent and accumulate in ecosystems, humanity’s growing reliance on metal resources compels the development of new tools to investigate metal toxicology. Elucidation of biologically relevant heavy metal binding motifs is fundamentally critical to toxicological assessment. My research group is specifically interested in the development of new NMR methods for toxicological assessment.
An estimated 30-50% of all proteins require interaction with a metal ion for full physiological activity. In general, metal ion binding sites are formed by the cooperative, simultaneous interaction of a number of ligating protein groups leading to considerable metal specificity. However, when challenged with non-essential metal ions, it is possible to replace native metal ions leading to physiologically detrimental changes in protein activity.
Cd(II), Hg(II) and Pb(II) are toxic metal ions which often dramatically alter the activity of enzymes and possess one or more isotope with favorable NMR properties. To begin development of NMR methods that can be used for toxicological assessment, the coordination chemistry of polydentate ligands containing donors which simulate ligation by amino acid side chain donors are being characterized with these metals by multinuclear NMR. In addition, we have recently begun to investigate the mercury coordination properties of cyclic dipeptides of histidine and methionine. The goal of these studies is to elucidate patterns in both chemical shifts and coupling constants that can be correlated with the geometry, coordination number, and identity of ligands in the primary coordination sphere of of these metals.
In the past twelve years, over 50 new complexes of Hg(II) involving fifteen different ligand systems have been prepared in my lab and structurally characterized by X-ray crystallography (through collaborations with Marj Kastner at Bucknell University, Raymond Butcher at Howard University and Robert Pike internally). Most of these complexes have been correlated with solution state NMR data. Significantly, many of these complexes have demonstrated slow-exchange behavior in solution on the NMR time scale which has rarely been observed for small coordination compounds of Hg(II). Furthermore, we have been able to measure heteronuclear coupling constants between 199Hg and 1H which are comparable in magnitude to those measured for mercury-substituted proteins. We have also conducted comparable studies with a number of Cd(II) complexes.
In addition to the mercury coordination chemistry studies, my research group recently began investigating the catalytic potential of silica immobilized copper complexes. At this time, the effect of the pH of immobilization on peroxidase and catechol oxidase activity is under investigation.
Selected Research Highlights:
Divergent Peptidyl alpha-Hydroxylating Monooxygenases”
“Investigation of Mechanism-Based Inhibition of Peptidyl
Monooxygenase” (with undergraduate Rebecca L. Casaubon)
“Structure-Spectroscopy Correlations of Multidentate Hg(II)
“Development of a Novel Probe of Metal-Protein Interactions
“Novel 199Hg NMR Methods for Monitoring Protein
“Investigation of Silica Supported Copper Complexes as
“Extending the Reach of 199Hg NMR as a
“Exploring the metal biochemistry of Hg(II) with multidentate
N,S-donor ligands by X-ray crystallography and NMR Spectroscopy”
“Foundations for Toxicological Studies: Probing Speciation and
of Cd(II), Hg(II) and Pb(II) with Multidentate Chelating Ligands”
Bold = Undergraduate; Italics = Master's Student
D. C. Bebout, D. E. Ehmann, J. C. Trinidad, K. K. Crahan, M. E. Kastner, and D. A. Parrish “Preparation of Hg(II) Complexes of Tris[(2-pyridyl)-methyl]amine and Characterization by X-ray Crystallography and NMR Spectroscopy” Inorg. Chem. 1997, 36, 4257-4264.
D. C. Bebout, D. E. Ehmann, A. E. DeLanoy, M. E. Kastner, D. A. Parrish, R. J. Butcher “Characterization of Hg(II) Complexes of Bis[(2-pyridyl)methyl]amine and by X-ray Crystallography and NMR Spectroscopy” Inorg. Chem.1998, 37, 2952-2959.
D. C. Bebout, J. F. Bush II, K. K. Crahan, M. E. Kastner, D. A. Parrish “Correlation of a Solution State Conformational Change between Mercuric Chloride Complexes of Tris[(2-(6-methylpyridyl))-methyl]amine with X-ray Crystallographic Structures” Inorg. Chem. 1998, 37, 4641-4646.
D. C. Bebout, S. W. Stokes, R. J. Butcher “Comparison of Heteronuclear Coupling Constants for Isostructural Nitrogen Coordination Compounds of 111/113Cd and 199Hg”, Inorg. Chem. 1999, 38, 1126-1133.
D. C. Bebout, J. F. Bush II, K. K. Crahan; E. V. Bowers, R. J. Butcher “Sterically Demanding Multidentate Ligand Tris[(2-(6-methylpyridyl)methyl]amine Slows Exchange and Enhances Solution State Ligand Proton NMR Coupling to 199Hg(II)” Inorg. Chem. 2002, 41, 2529.
D. C. Bebout, J. F. Bush II, E. M. Shumann, J. A. Viehweg, M. E. Kastner; D. A. Parrish, S. M. Baldwin “Caging the Mercurous Ion: A Tetradentate Tripodal Nitrogen Ligand Enhances Stability and J(1H199Hg),” J. Chem. Cryst. 2003, 33, 455-462.
D. C. Bebout, G. S. Murphy, M. M. Garland, E. V. Bowers, R. J. Butcher “Investigation of the Mercury(II) Coordination Chemistry of Tris[(1-methylimidazol-2-yl)methyl]amine by X-ray Crystallography and NMR,” Dalton Trans. 2003, 2578-2584.
D. C. Bebout “Mercury: Inorganic and Coordination Chemistry” Encyclopedia of Inorganic Chemistry, 2nd edition, 2005, in print.
S. M. Berry, D. C. Bebout, R. J. Butcher “Solid state and solution state coordination chemistry of the zinc triad with the mixed N,S donor ligand bis(2-methylpyridyl)sulfide” Inorg. Chem. 2005, 44, 27-39.
M. M. Makowska-Grzyska, K. Doyle, R. A. Allred, E. Szajna, A. M. Arif, D. C. Bebout and L. M. Berreau “Structural, Spectroscopic and Reactivity Properties of N2S2(thioether)O(amide)-Ligated Hg(II) Complexes: The First Examples of Hg(II)-mediated Amide Cleavage,” Eur. J. Inorg. Chem. 2005, 822-827.
W. Lai, S. M. Berry, D. C. Bebout and R. J. Butcher “Investigation of Group 12 Metal Complexes with a Tridentate SNS Ligand by X-ray Crystallography and 1H NMR” Inorg. Chem. accepted pending revisions.
D. C. Bebout and S. M Berry "Probing Mercury Complex Speciation with Multinuclear NMR" Structure and Bonding: Recent Developments in Mercury Science, manuscript in preparation.
W. Lai, S. M. Berry, D. C. Bebout and R. J. Butcher “
Comparison of Zinc Triad Metal Complexes of
N-(2-pyridylmethyl)-N-(2-(methylthio)ethyl)amine in the Solid- and
Solution-State", manuscript in preparation.
Additional courses no longer offered by the
Steven M . Berry
Camille & Henry Dreyfus Postdoctoral Fellow
University of Minnesota - Duluth 1997
PhD, University of Illinois at Urbana -
Dr. Steven M. Berry joined the Bebout research group in June 2003 as a prestigious Camille & Henry Dreyfus Postdoctoral Fellow. Dr. Berry investigated the coordination chemistry of the zinc triad with mixed N,S-donor ligands. He prepared over ten new complexes that have been characterized crystallographically in collaboration with Raymond Butcher at Howard University. Most of these complexes have also been characterized in the solution state by multinuclear NMR. His work in the Bebout lab contributed to one published paper and two more that are in preparation. He also contributed to a an invited review article for the Structure and Bonding series.
At The College of William and Mary, Dr. Berry taught a section of Chem 151: Chemistry Laboratory I (General) in Fall 2003 and cotaught a section of Chem 420: Biochemistry Laboratory with Professor Lisa Landino in Spring 2004 and 2005. He cotaught a new section of Chem 414: Biochemistry tailored to chemistry majors with Professor Bebout during Spring 2004 and 2005. He taught a section of Chem 103: General Chemistry I.
Dr. Berry started a tenure-track faculty position in the
Department at the University of Minnesota - Duluth in fall 2005.