Courses | Christopher A. Del Negro

Fall 2009:

Applied Cellular Neuroscience (apsc 431/631).

Here we examine cellular neurophysiology including membrane potentials, ion channels and membrane permeability, electrical signaling and cable properties, synaptic transmission, neuromodulation, and second messenger systems. We apply these concepts to motor control, homeostatic regulation, special senses.

Course Outline:

[27.AUG.2009] spectrum of cellular properties in neurons - - - passive electrical properties and RC circuits - - -
Fain 1-38, Hille 1-13 | Lecture 1

[01.SEP.2009] the cable equation - - - cable theory and synaptic transmission - - -
Fain 38-62 | Lecture 2

[03.SEP.2009] ion permeability and membrane potentials - - - Nernst-Planck Equation - - - current-voltage relations - - - chord conductance equation - - -
Fain 63-72, Hille 13-22 | Lecture 3

[08.SEP.2009] Goldman-Hodgkin-Katz (GHK) current equation - - - Goldman rectification - - - GHK voltage equation and calculating the resting membrane potential for multiple permeant ions - - -
Fain 72-94, Hille 309-319 | Lecture 4

[10.SEP.2009] Applications of the GHK voltage equation - - - rectification of receptor currents - - - estimating ion selectivity - - - Na/K ATPase pump current - - - pumps contribute to resting membrane potential - - -
Fain 79-109 | Lecture 5

[15.SEP.2009] physiological effects of electrogenic Na/K ATPase pumps - - - calcium sequestration, buffering, and extrusion - - - electrogenicity of Na-Ca cotransport (exchange) - - - chloride distribution across excitable cell membranes - - - bicarbonate distributions - - - regulation of pH - - -
Fain 109-127, Kirichok et al. Nature 427: 360-4, 2004 | Lecture 6

[17.SEP.2009] mitochondrial calcium channels and rectification - - - review of Homework 3 - - -
Kirichok et al. Nature 427: 360-4, 2004 | Lecture 7

[22.SEP.2009] Classical biophysics of squid axon - - - ionic basis of action potentials - - - Hodgkin-Huxley equations - - -
Fain 131-170, Hille 25-60 | Lecture 8

[24.SEP.2009] Voltage-dependent inactivation (sodium channels are partially inactivated at rest!) - - - fitting Hodgkin-Huxley equationss to data - - - simulating the action potential - - - anode-break excitation (post-inhibitory rebound) - - -
Fain 131-170, Hille 25-60 | Lecture 9

[29.SEP.2009] Structure and function of voltage-gated channels - - -
Fain 171-207, Hille 61-93 | Lecture 10

[01.OCT.2009] Diversity of voltage-gated channels - - - Na+ channels, persistent Na⁺ current and modal gating - - -
Fain 208-252, Hille 95-167 | Lecture 11

[06.OCT.2009] Diversity of voltage-gated channels - - - Ca²⁺ channels, high-threshold, low-threshold, and their separation - - -
Fain 208-252, Hille 95-167 | Lecture 12

[08.OCT.2009] Presynaptic mechanisms of synaptic transmission - - - the Ca²⁺ hypothesis - - - input-output relationships - - -
Fain 255-307 | Lecture 13

[15.OCT.2009] Quantal nature of synaptic transmission - - - mini analysis of synaptic transmission - - - pre- versus post-synaptic measurements - - -
Fain 269-307 | Lecture 14

[22.OCT.2009] excitatory synaptic transmission - - - nicotinic acetylcholine receptors - - - ionotropic glutamate receptors (AMPA, Kainate, NMDA) - - - cumulative probability mEPSC histograms - - -
Fain 309-359 | Lecture 15

[27.OCT.2009] inhibitory synaptic transmission - - - GABA and glycine receptors - - - pharmacology (barbiturates / benzodiazipines) - - - mulitple conductance states - - - presynaptic inhibition - - -
Fain 357-382 | Lecture 16

[29.OCT.2009] metabotropic synaptic transmission - - - families of G-protein-coupled receptors - - - effectors - - - second messengers - - - targets - - - neuromodulation and beta-adrenergic actions on Ca²⁺ current in heart - - -
Fain 385-406, Hille 201-211 | Lecture 17

[03.NOV.2009] G-protein-coupled receptors and desensitization - - - adenylyl cyclase, cAMP, and protein kinase A (PKA) - - - membrane delimited modulation by beta-gama subunits - - - §-adrenergic + muscarinic (cholinergic) modulation of cardiac rate and contractility - - - pleitropy of G-protein signaling - - -
Fain 407-415, 423-429, Hille 212-236 | Lecture 18

[05.NOV.2009] G-protein modulation of Ca²⁺ channels - - - phospholipases and protein kinase C - - - mGluR6 and ON bipolar cells - - -
Fain 416-423, 429-439 Morgans et al. PNAS, 2009 | Lecture 19

[10.NOV.2009] mGluR6 and ON bipolar cells - - - Ca²⁺ as a second messenger - - - Ca²⁺ buffers and indicator dyes - - - intracellular Ca²⁺ release mechanisms - - -
Fain 440-458, Svoboda & Yasuda. Neuron, 2006 | Lecture 20

[12.NOV.2009] intracellular Ca²⁺ release mechanisms - - - Ca²⁺ waves - - - 'CRAC' current - - - CaM Kinase II - - - nitric oxide (NO) - - -
Fain 458-475 | Lecture 21

[17.NOV.2009] long-term potentiation - - - NMDA-dependent LTP - - - structure of the hippocampus and tri-synaptic circuit - - - CaM Kinase II and AMPA receptors - - - 'silent' synapses - - -
Fain 476-498 | Lecture 22

[19.NOV.2009] long-term depression (LTD) - - - NMDA receptor-independent LTP - - - mechanotransduction - - - hair cells of the cochlea - - -
Fain 498-end of Ch. 14, Ch. 15 | Lecture 23

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Associate Professor
Department of Applied Science
McGlothlin-Street Hall, Rm. 318
The College of William and Mary
Williamsburg, Virginia 23187-8795

Office/Lab: 757.221.7808
Fax: 757.221.2050
E-mail: cadeln@wm.edu
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